Resurrecting the Past

Dire Wolves Return and Spark Scientific Debate

In April 2025, a headline in The New York Times proclaimed, “Scientists Revive the Dire Wolf or Something Close,” with news that Colossal Laboratories and Biosciences, a deextinction company, had essentially brought an apex predator back to life.

Using ancient, preserved DNA from a dire wolf fossil, paired with that of a gray wolf, Colossal engineered the rebirth of a species that last roamed North and South America more than 12,000 years ago. Three pups named Remus, Romulus and Khaleesi (the latter after a popular character in the HBO TV series, “Game of Thrones”) were born via surrogate and live in captivity at an undisclosed wildlife facility in the United States.

The news ignited considerable discussion within the scientific community and beyond. When Julie Meachen, Ph.D., professor of anatomy at Des Moines University Medicine and Health Sciences, heard news of the dire wolf’s return to civilization, she was skeptical. For several years, her work has centered around the study of wolves, particularly dire wolves. While the public response was largely enthusiastic, Meachen remained cautiously optimistic regarding the implications of such ambitious rewilding efforts.

“I’m not a huge fan of rewilding in general, at least not of Pleistocene species,” says Meachen, whose expertise lies in the paleoecology, anatomy and morphology of Ice Age species. “It’s not a straightforward thing to do, and it takes a lot of energy and money to do something like this, so I wanted to know why.”

Meachen recalls that two years earlier, a friend, Beth Shapiro, Colossal’s chief scientist, reached out with questions about the best methods for gathering ancient DNA, extracting bone samples without damaging invaluable skull specimens and acquiring permissions to sample a specimen. Despite her input, Meachen was kept in the dark about Colossal’s ultimate goal of creating live dire wolves until approximately a week before the public announcement.

“I had an inkling,” she says. “It’s Colossal. I knew what they were up to. I know what their ultimate goal is,” she says, but emphasizes that the company never explicitly confirmed their intention to clone dire wolves.

Moreover, Meachen believes that while closely related, their creation is not a true dire wolf. “I don’t think they are actually dire wolves,” Meachen told ABC News in April. “What we had is something new — we have a mostly gray wolf that looks like a dire wolf.”

A modern coyote skull is surrounded by fossil gray wolf (Beringian wolf) bones from the Natural Trap Cave. Additional bones, starting in the upper right, include: a partial ulna, calcaneum, astragalus (talus) and mandibular fragment.
A modern coyote skull is surrounded by fossil gray wolf (Beringian wolf) bones from the Natural Trap Cave. Additional bones, starting in the upper right, include: a partial ulna, calcaneum, astragalus (talus) and mandibular fragment.

The speed of the project was particularly striking to Meachen. It took less than two years from the initial DNA collection in May 2023 to the announcement of the dire wolves’ creation. “That’s incredibly fast for science,” she says. “A year and eight months is unheard of.”

Looking ahead, Colossal has hinted at other species for de-extinction, including the thylacine (also known as the marsupial wolf), the dodo and the woolly mammoth. Meachen expressed enthusiasm for the thylacine and the passenger pigeon, both of which went extinct relatively recently, making their reintegration into ecosystems potentially more feasible. However, she remains cautious about the woolly mammoth, questioning where such a large animal would live in a world increasingly dominated by human activity

Focus on Conservation and Funding for Research

Beyond the spectacle of de-extinction, Meachen highlights Colossal’s concurrent efforts in conservation. The same genetic techniques used for bringing back extinct animals are being employed to introduce genetic variation into living species facing extinction due to habitat loss and reduced genetic diversity. This application, she believes, holds significant promise for bolstering endangered populations.

Meachen has concerns about the broader implications of declining research funding in the United States. Cutting funding for scientific research, particularly “research for research’s sake,” undermines the nation’s capacity for technological advancement and problem-solving, she says.

“You can’t just look at a piece of research and say, ‘that’s useless’ because you don’t actually know what’s going to come from it,” she emphasizes. She fears that underfunding science will lead to a brain drain, with scientists seeking opportunities in countries like China, which heavily invests in research. This could leave the U.S. uncompetitive.

Julie Meachen, Ph.D., rappels 80 feet into the Natural Trap Cave in northern Wyoming. The chamber itself only measures 140 feet in diameter.
Julie Meachen, Ph.D., rappels 80 feet into the Natural Trap Cave in northern Wyoming. The chamber itself only measures 140 feet in diameter. Photograph by Justin Sipla.

Outside of the Box

As for her own work, Meachen continues to dedicate her career to learning all she can about Ice Age species. Her ongoing research at the Natural Trap Cave fossil site in northern Wyoming was recently featured in the June issue of Smithsonian magazine and showcases her passion for paleontology, particularly for ancient carnivores. Meachen says her interest stems from her undergraduate days, where she was captivated by saber-toothed cats and eventually pursued a Ph.D. focusing on big carnivores. Her unique blend of expertise in anatomy and ancient animals makes her a valuable voice in the landscape of de-extinction and conservation science.

It is also applicable to other areas of science, says Lindsay Mahaney, D.O.’26. Mahaney was one of several DMU students who’ve worked with Meachen in the field, first at Rancho La Brea tar pits and later at the Natural Trap Cave in Wyoming. The research, she admits, honed her attention to detail and investigative skills, crucial for her career path from forensic anthropology to forensic pathology and eventually to OB-GYN.

A scene from the Natural Trap Cave, which is home to the remains of several Ice Age mammals.
The Natural Trap Cave is home to the remains of several Ice Age mammals. Photograph by Justin Sipla.
Lindsay Mahaney. D.O.’26, on her first trip to the Natural Trap Cave in 2021. Photograph by Mike Fallon
Lindsay Mahaney. D.O.’26, on her first trip to the Natural Trap Cave in 2021. Photograph by Mike Fallon.

“The way I see it, researchers and doctors are pretty similar in that we have to have a very systematic way of thinking. We have a question that we’re trying to answer, and then we look at all the evidence in front of us to find out what the answer could be,” Mahaney says. “When I first started working with Dr. Meachen, I didn’t know that I wanted to go to medical school. I thought that I wanted to go into forensic anthropology, but I didn’t realize at the time that a lot of the skills that I did learn through that research would actually play a pretty important role to what I do a lot now with clinical sciences.”

Mahaney says Meachen’s influence and mentorship will benefit her for years to come, and she’s grateful for the experience.

“She’s taught me so much,” Mahaney says. “After years of doing this, I can usually look at a bone and be like, ‘Okay, this was probably an herbivore because of the shape of the tooth,’ or ‘It was a molar from probably this animal.’ She (Meachen) can take a look at a little piece of bone and say, ‘Oh, it’s probably this cranial bone from this era, from this sub-species of something,’ and I just think that that’s incredible.”

Research Powerhouse

DMU maintains a robust research enterprise, actively driving innovation and contributing to advancements in medical science and education. Meachen’s story is just one powerful example of the groundbreaking work and thinking happening here.

It’s not every day that a scientist and professor from Iowa makes the national news, but that’s exactly what happened to Julie Meachen this spring.

When the news broke about the dire wolf pups, Meachen, who co-authored a 2021 research paper on the evolutionary history of the dire wolf, was the person media outlets like The Washington Post, The New York Times, ABC News, Science News and even Rolling Stone turned to for a quote.

Julie Meachen joined Iowa Public Radio for an on-air interview.
Julie Meachen is interviewed by Des Moines’ ABC affiliate station.

Colossal Biosciences said it restored the long-extinct canine species.

“Is this for purely entertainment purposes? The mission of helping to preserve the species that are alive and save them from the brink of extinction is an incredibly admirable mission. That is a mission that I could get behind 100%.”

— ABC News, April 7, 2025

Dire wolves, made famous by “Game of Thrones,” went extinct some 13,000 years ago. Now, researchers have bred gray wolf pups that carry genes of their ancient cousins.

Dr. Meachen, who was not involved in the creation of the wolf pups, said that she had mixed feelings about the de-extinction effort. “All the little-kid feelings in me say that I want to see what they look like,” she said. “But I have questions. We have trouble with the wolves we have today.”

— The New York Times, April 7, 2025

Pioneers in the science of “de-extinction,” an American company has announced the births of three pups whose genes resemble those of a species that hasn’t roamed Earth for millennia

“We have a mostly gray wolf that looks like a dire wolf,” Julie Meachen, a vertebrate paleontologist from Des Moines University who was not involved in the project, says to ABC News. Meachen co-authored a paper on the evolution of dire wolves along with Shapiro in 2021, which found that the species is genetically distinct from gray wolves, having diverged from the wolf lineage nearly six million years ago.

As for these new creatures, Meachen adds, “I don’t think they are actually dire wolves.”

— Smithsonian Magazine, April 8, 2025

Genetic tools used to create the fluffy, white pups could be used to help at-risk animals

Across the internet and social media, critics of Colossal’s work have been vocal about dire wolves not actually being wolves at all. That argument seems to be based largely on a 2021 Nature paper that attempted to reconstruct the animals’ evolutionary history. After analyzing the ancient genomes of five dire wolves, researchers concluded that though the animals looked similar to gray wolves, they were highly different, forging separate evolutionary paths nearly 6 million years ago.

At the time, “we thought that there was enough genetic divergence that they should not be in the genus Canis,” says Julie Meachen, a vertebrate paleontologist at Des Moines University in Iowa. Canis is the group that includes wolves, dogs and coyotes.

— Science News, April 10, 2025

Meachen was not involved in creating the wolf pups but provided DNA samples collected from animal remains in Idaho and Ohio to help fine-tune the deextinction project.

The dire wolf project would have been possible without her input, but would not have been as accurate, she tells Axios.

— Axios, April 10, 2025

By Jody Gifford  |  Photographs By Paul Joy and Colossal Biosciences

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